Orvosi diagnosztikai eszközök, labor műszerek,
reagensek beszerzése és forgalmazása

Catalog Number: ACI 3180 A, B API 3180 AA
Description: 0.1, 0.5 ml, 6.0 ml, prediluted
concentrated
Dilution: 1:100 Ready-to-use
Diluent: Renoir Red N/A

IVD - CE

Intended Use:
For In Vitro Diagnostic Use
IMP3 (RM) [EP286] is a rabbit monoclonal antibody that is intended for laboratory use in the qualitative identification of IMP3 protein by immunohistochemistry (IHC) in formalin-fixed paraffin-embedded (FFPE) human tissues. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.

Summary and Explanation:
IMP3 (insulin-like growth factor II mRNA-binding protein 3) is a 580 amino acid oncofetal RNA binding protein containing four K homology domains which is encoded by a 4350 bp mRNA transcript produced by the IGF2BP3 gene on chromosome 7p11.5 (1,2). Its relevance as a novel biomarker in many kinds of cancer has been recently published and data also suggest that IMP3 may play an important role in malignant transformation (3). Cytoplasmic expression of IMP3 has been associated with a more aggressive phenotype in many cancers including triple negative (basal-like) breast cancers, colon cancers, lung cancer and prostate cancer (4-7). IMP3 has also been used in discriminating between benign vs malignant cancers and IMP3 has been shown to be a marker of high-grade dysplasia in esophageal adenocarcinoma (3, 8-10).

Principle of Procedure:
Antigen detection in tissues and cells is a multi-step immunohistochemical process. The initial step binds the primary antibody to its specific epitope. After labeling the antigen with a primary antibody, an enzyme labeled polymer is added to bind to the primary antibody. The detection of the bound antibody is evidenced by a colorimetric reaction.
Source: Rabbit monoclonal
Species Reactivity: Human; others not tested
Clone: EP286
Isotype: IgG


Total Protein Concentration: ~10 mg/ml. Call for lot specific Ig concentration.

Epitope/Antigen: A synthetic peptide corresponding to the human IMP3 protein

Cellular Localization: Cytoplasmic/nuclear
Positive Tissue Control: Lung squamous carcinoma and normal pancreas

Known Applications:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)

Supplied As: Buffer with protein carrier and preservative

Storage and Stability:
Store at 2ºC to 8ºC. Do not use after expiration date printed on vial. If reagents are stored under conditions other than those specified in the package insert, they must be verified by the user. Diluted reagents should be used promptly; any remaining reagent should be stored at 2ºC to 8ºC.

Protocol Recommendations:

Peroxide Block:
Block for 5 minutes with Biocare's Peroxidazed 1.

Pretreatment: Perform heat retrieval using Biocare’s Diva Decloaker. Refer to the Diva Decloaker data sheet for specific instructions.

Protein Block (Optional): Incubate for 5-10 minutes at RT with Biocare's Background Punisher.

Primary Antibody: Incubate for 60 minutes at RT.
Probe: N/A

Polymer: Incubate for 30 minutes at RT with a secondary-conjugated polymer.

Chromogen: Incubate for 5 minutes at RT with Biocare’s DAB – OR – Incubate for 5-7 minutes at RT with Biocare’s Warp Red.

Counterstain:
Counterstain with hematoxylin. Rinse with deionized water. Apply Tacha's Bluing Solution for 1 minute. Rinse with deionized water.