Orvosi diagnosztikai eszközök, labor műszerek,
reagensek beszerzése és forgalmazása

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Víztelenítés
BioCare akció 2021
Koronavírus diagnosztika
Patológia
Flow citometria
Klinikai kémia
Mikrobiológia
Transzfuziológia
Transzplantációs immungenetika
Sejtszeparálás
Biokémia
Stemgent
Kontrasztanyagok
Klinikai alkalmazások
Allergológia

Frank Diagnosztika Kft.
1031 Budapest, Szentendrei út 176.
+36 1 250-1813
frankdiagn@frank-diagn.hu



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Patológia
Műszerek és eszközök
AUS140 Festőautomata
Gyártó: Bio-Optica
AUS140 festőautomata

A Bio-Optica AUS 140 festőautomata teljes megoldást kínál a hisztológiai és citológiai metszetek rutin és speciális festéséhez, folyamatos betöltési lehetőséggel (30 tárgylemezes kosarak) a rugalmas üzemeltetés érdekében.
Önálló asztali egység összesen 28 vegyszerálló reagens állomással, beépített kemencével vagy nélküle (kívánság szerint), 5 mosó állomással amelyekben áramlik a mosóvíz (nyomás szabályozás és áramlás időzítés), 2 berakodó és 3 kirakodó állomás, 2 forró levegős szárító állomás.
Több mint 2000 tárgylemez / nap kapacitás, a festési eljárás random módon kijelölhető, akár 10-12 párhuzamosan futó festési program.
Integrált aktív szenes szűrő a keletkező káros vegyszergőzök (xilol, alkohol) elszívására és semlegesítésére, érintőképernyős monitor, egyszerű kezelés, több mint 50 különböző festési protokoll tárolási lehetősége. Beépített áramforrás váratlan áramszünet rövidtávú (30 perces) áthidalására.

Kompatibilis Sakura, Shandon, Leica és Medite kosarakkal.

Külső méretek: 1150 x 770 x 900 mm (Hossz x Szélesség x Magasság) + 400 mm a monitor
Katalógusszám: 40-400-200 AUS140 kemence nélküli
40-400-250 AUS140 kemencével

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AUS140 Festőautomata
Gyártó: Bio-Optica
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Gyártó: Biocare Medical
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Ki-67 + pHH3
Gyártó: Biocare Medical

Catalog Number: API 3198DS AA
Description: 6.0 ml, prediluted
Dilution: Ready-touse
Diluent: N/A

Intended Use:
For In Vitro Diagnostic Use
Ki-67 + pHH3 is a cocktail of mouse and rabbit monoclonal antibodies that is intended for laboratory use in the qualitative identification of Ki-67 and phosphohistone H3 (Ser10) proteins by immunohistochemistry (IHC) in formalin-fixed paraffin-embedded (FFPE) human tissues. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.
Summary and Explanation:
The Ki-67 nuclear antigen is associated with cell proliferation and found throughout the cell cycle; though not in G0 phase (1,2). The assessment of Ki-67 proliferation in breast cancers has shown the Ki-67 labelling index is an important predictor of survival (3).
Microscopic evaluation of mitotic figures on H&E is a routine procedure in the assessment of the tumor grades (4). However, the counting of mitosis is manual and time consuming with assorted difficulties as well as variabilities between interobserver assessments (5). Histone H3 phosphorylation at Serine10 (pHH3) is in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle. pHH3 can distinguish mitosis from apoptotic nuclei (6). The immunohistochemical staining of Serine10-pHH3 has been reported to be comparable to mitotic figures in the H&E section (7-10).
Immunohistochemical studies have shown immunostaining using anti- Ki-67 and anti-pHH3 antibodies provided improved prediction of recurrence of tumors and may become effective ancillary tools in deciding on optimal therapeutic management (11). Other studies have shown Ki-67 and pHH3 can be useful in separating malignant melanoma from benign nevi (12).
Principle of Procedure:
This product is a primary antibody cocktail of mouse and rabbit antibodies, which may be used in a Multiplex IHC staining procedure to produce a two-color stain. Following application of the primary antibody cocktail to the tissue
sample, detection is performed by separate secondary antibodies specific for each species (i.e. mouse or rabbit)
of the primary antibody cocktail, which are conjugated to horseradish peroxidase (HRP) or alkaline phosphatase (AP) enzymes. Visualization is accomplished by the application of chromogenic substrates (DAB and Warp Red), which are enzymatically activated (by HRP or AP, respectively) to produce a colored reaction product at the antigen site. The specimen may be counterstained and coverslipped. Results are interpreted using a light microscope.

Reagent Provided:
Ki-67 + pHH3 is provided as a prediluted antibody cocktail of anti- Ki-67 and anti-pHH3 antibodies in buffer with carrier protein and preservative.

Antibody anti-Ki-67 anti-pHH3
Clone MIB-1 BC37
Source Mouse monoclonal Rabbit monoclonal
Isotype IgG/kappa IgG
Epitope/ Antigen 1002 bp Ki-67 cDNA fragment PhosphoSer10 of Histone H3
Cellular Localization Nuclear Nuclear (mitotic figure)
Staining Red
(Warp Red) Black (Deep Space)

Storage and Stability:
Store at 2°C to 8°C. Do not use after expiration date printed on vial. If reagents are stored under conditions other than those specified in the package insert, they must be verified by the user. Diluted reagents should be used promptly; any remaining reagent should be stored at 2°C to 8°C.
Known Applications:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
Species Reactivity: Human; others not tested
Positive Tissue Control: Melanoma or colon cancer
Protocol Recommendations:
Peroxide Block:
Block for 5 minutes with Biocare's Peroxidazed 1.
Pretreatment: Perform heat retrieval using Biocare’s Diva Decloaker. Refer to the Diva Decloaker data sheet for specific instructions.
Protein Block: Incubate for 10 minutes at RT with Biocare's Background Punisher.
Primary Antibody: Incubate for 30 minutes at RT.
Double Stain Detection: Incubate for 30 minutes at RT using Biocare's MACH 2 Double Stain 1.
Chromogen (1): Incubate for 5 minutes at RT with Biocare's Deep Space Black.
Chromogen (2): Incubate for 5-7 minutes at RT with Biocare's Warp Red. Rinse in deionized water.
Counterstain:
Counterstain with hematoxylin. Rinse with deionized water. Apply Tacha's Bluing Solution for 1 minute. Rinse with deionized water.



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IMP3 (RM)
Gyártó: Biocare Medical
Catalog Number: ACI 3180 A, B API 3180 AA
Description: 0.1, 0.5 ml, 6.0 ml, prediluted
concentrated
Dilution: 1:100 Ready-to-use
Diluent: Renoir Red N/A

IVD - CE

Intended Use:
For In Vitro Diagnostic Use
IMP3 (RM) [EP286] is a rabbit monoclonal antibody that is intended for laboratory use in the qualitative identification of IMP3 protein by immunohistochemistry (IHC) in formalin-fixed paraffin-embedded (FFPE) human tissues. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.

Summary and Explanation:
IMP3 (insulin-like growth factor II mRNA-binding protein 3) is a 580 amino acid oncofetal RNA binding protein containing four K homology domains which is encoded by a 4350 bp mRNA transcript produced by the IGF2BP3 gene on chromosome 7p11.5 (1,2). Its relevance as a novel biomarker in many kinds of cancer has been recently published and data also suggest that IMP3 may play an important role in malignant transformation (3). Cytoplasmic expression of IMP3 has been associated with a more aggressive phenotype in many cancers including triple negative (basal-like) breast cancers, colon cancers, lung cancer and prostate cancer (4-7). IMP3 has also been used in discriminating between benign vs malignant cancers and IMP3 has been shown to be a marker of high-grade dysplasia in esophageal adenocarcinoma (3, 8-10).

Principle of Procedure:
Antigen detection in tissues and cells is a multi-step immunohistochemical process. The initial step binds the primary antibody to its specific epitope. After labeling the antigen with a primary antibody, an enzyme labeled polymer is added to bind to the primary antibody. The detection of the bound antibody is evidenced by a colorimetric reaction.
Source: Rabbit monoclonal
Species Reactivity: Human; others not tested
Clone: EP286
Isotype: IgG


Total Protein Concentration: ~10 mg/ml. Call for lot specific Ig concentration.

Epitope/Antigen: A synthetic peptide corresponding to the human IMP3 protein

Cellular Localization: Cytoplasmic/nuclear
Positive Tissue Control: Lung squamous carcinoma and normal pancreas

Known Applications:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)

Supplied As: Buffer with protein carrier and preservative

Storage and Stability:
Store at 2ºC to 8ºC. Do not use after expiration date printed on vial. If reagents are stored under conditions other than those specified in the package insert, they must be verified by the user. Diluted reagents should be used promptly; any remaining reagent should be stored at 2ºC to 8ºC.

Protocol Recommendations:

Peroxide Block:
Block for 5 minutes with Biocare's Peroxidazed 1.

Pretreatment: Perform heat retrieval using Biocare’s Diva Decloaker. Refer to the Diva Decloaker data sheet for specific instructions.

Protein Block (Optional): Incubate for 5-10 minutes at RT with Biocare's Background Punisher.

Primary Antibody: Incubate for 60 minutes at RT.
Probe: N/A

Polymer: Incubate for 30 minutes at RT with a secondary-conjugated polymer.

Chromogen: Incubate for 5 minutes at RT with Biocare’s DAB – OR – Incubate for 5-7 minutes at RT with Biocare’s Warp Red.

Counterstain:
Counterstain with hematoxylin. Rinse with deionized water. Apply Tacha's Bluing Solution for 1 minute. Rinse with deionized water.

bővebben »

IMP3 (RM)
Gyártó: Biocare Medical
Catalog Number: ACI 3180 A, B API 3180 AA
Description: 0.1, 0.5 ml, 6.0 ml, prediluted
concantrated
Dilution: 1:100 Ready-to-use
Diluent: Renoir Red N/A

IVD - CE

Intended Use:
For In Vitro Diagnostic Use
IMP3 (RM) [EP286] is a rabbit monoclonal antibody that is intended for laboratory use in the qualitative identification of IMP3 protein by immunohistochemistry (IHC) in formalin-fixed paraffin-embedded (FFPE) human tissues. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.

Summary and Explanation:
IMP3 (insulin-like growth factor II mRNA-binding protein 3) is a 580 amino acid oncofetal RNA binding protein containing four K homology domains which is encoded by a 4350 bp mRNA transcript produced by the IGF2BP3 gene on chromosome 7p11.5 (1,2). Its relevance as a novel biomarker in many kinds of cancer has been recently published and data also suggest that IMP3 may play an important role in malignant transformation (3). Cytoplasmic expression of IMP3 has been associated with a more aggressive phenotype in many cancers including triple negative (basal-like) breast cancers, colon cancers, lung cancer and prostate cancer (4-7). IMP3 has also been used in discriminating between benign vs malignant cancers and IMP3 has been shown to be a marker of high-grade dysplasia in esophageal adenocarcinoma (3, 8-10).

Principle of Procedure:
Antigen detection in tissues and cells is a multi-step immunohistochemical process. The initial step binds the primary antibody to its specific epitope. After labeling the antigen with a primary antibody, an enzyme labeled polymer is added to bind to the primary antibody. The detection of the bound antibody is evidenced by a colorimetric reaction.
Source: Rabbit monoclonal
Species Reactivity: Human; others not tested
Clone: EP286
Isotype: IgG


Total Protein Concentration: ~10 mg/ml. Call for lot specific Ig concentration.

Epitope/Antigen: A synthetic peptide corresponding to the human IMP3 protein

Cellular Localization: Cytoplasmic/nuclear
Positive Tissue Control: Lung squamous carcinoma and normal pancreas

Known Applications:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)

Supplied As: Buffer with protein carrier and preservative

Storage and Stability:
Store at 2ºC to 8ºC. Do not use after expiration date printed on vial. If reagents are stored under conditions other than those specified in the package insert, they must be verified by the user. Diluted reagents should be used promptly; any remaining reagent should be stored at 2ºC to 8ºC.

Protocol Recommendations:

Peroxide Block:
Block for 5 minutes with Biocare's Peroxidazed 1.

Pretreatment: Perform heat retrieval using Biocare’s Diva Decloaker. Refer to the Diva Decloaker data sheet for specific instructions.

Protein Block (Optional): Incubate for 5-10 minutes at RT with Biocare's Background Punisher.

Primary Antibody: Incubate for 60 minutes at RT.
Probe: N/A

Polymer: Incubate for 30 minutes at RT with a secondary-conjugated polymer.

Chromogen: Incubate for 5 minutes at RT with Biocare’s DAB – OR – Incubate for 5-7 minutes at RT with Biocare’s Warp Red.

Counterstain:
Counterstain with hematoxylin. Rinse with deionized water. Apply Tacha's Bluing Solution for 1 minute. Rinse with deionized water.

bővebben »

H.pylori (RM)
Gyártó: Biocare Medical
H. pylori (RM)
Prediluted Rabbit Monoclonal Antibody
901-3191-122216
Rev: 101716
Intended Use:
For In Vitro Diagnostic Use
H. pylori (RM) [EP279] is a rabbit monoclonal antibody that is intended
for laboratory use in the qualitative identification of H. pylori protein by
immunohistochemistry (IHC) in formalin-fixed paraffin-embedded
(FFPE) human tissues. The clinical interpretation of any staining or its
absence should be complemented by morphological studies using
proper controls and should be evaluated within the context of the
patient’s clinical history and other diagnostic tests by a qualified
pathologist.
Summary and Explanation:
Helicobacter pylori (H. pylori) infection represents the major cause of
peptic ulcer disease and is a likely contributing factor in the
development of gastric neoplastic diseases such as gastric
adenocarcinoma and mucosa-associated lymphoid tissue (MALT)
lymphoma (1). H. pylori are spiral-curved, gram-negative bacteria.
The majority of H. pylori microorganisms reside in the unstirred layer
of the gastric mucus, but some bind to the luminal aspect of gastric
foveolar cells, and attach at or near intercellular junctions, or even
locate within cells (2-3). However, generally they do not invade the
underlying lamina propria (3). Immunohistochemical (IHC) detection of
H. pylori can specifically distinguish the microorganisms from other
types of bacterial infections. Complete staining of the H. pylori
microrganism has been shown with the rabbit monoclonal. An IHC
technique, with heat-induced-antigen retrieval for detection of H. pylori
in gastric biopsies, is highly reliable, highly sensitive, specific, and can
be used on fully automated IHC platforms.
Principle of Procedure:
Antigen detection in tissues and cells is a multi-step
immunohistochemical process. The initial step binds the primary
antibody to its specific epitope. After labeling the antigen with a
primary antibody, an enzyme labeled polymer is added to bind to the
primary antibody. The detection of the bound antibody is evidenced by
a colorimetric reaction.
Source: Rabbit monoclonal
Species Reactivity: Human; others not tested
Clone: EP279
Isotype: IgG
Total Protein Concentration: ~10 mg/ml. Call for lot specific Ig
concentration.
Epitope/Antigen: H. pylori
Cellular Localization: Spiral shaped bacterium
Positive Tissue Control: Gastric tissue infected with H. pylori
Known Applications:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
Supplied As: Buffer with protein carrier and preservative
Storage and Stability:
Store at 2°C to 8°C. Do not use after expiration date printed on vial. If
reagents are stored under conditions other than those specified in the
package insert, they must be verified by the user.
Protocol Recommendations (manual use):
Peroxide Block:
Block for 5 minutes with Biocare's Peroxidazed 1.
Pretreatment: Perform heat retrieval using Biocare’s Borg Decloaker.
Refer to the Borg Decloaker data sheet for specific instructions.
Protein Block (Optional): Incubate for 5-10 minutes at RT with
Biocare's Background Punisher.
Primary Antibody: Incubate for 30 minutes at RT.
Probe: N/A
Polymer: Incubate for 30 minutes at RT with a secondary-conjugated
polymer.
Chromogen: Incubate for 5 minutes at RT with Biocare’s DAB – OR –
Incubate for 5-7 minutes at RT with Biocare’s Warp Red.
Counterstain:
Counterstain with hematoxylin. Rinse with deionized water. Apply
Tacha's Bluing Solution for 1 minute. Rinse with deionized water.
Protocol Recommendations (ONCORE Automated Slide
Staining System):
OAI3191 is intended for use with the ONCORE Automated Slide
Staining System. Refer to the ONCORE Automated Slide Staining
System User Manual for specific instructions on its use. Protocol
parameters in the ONCORE Automated Slide Stainer Protocol Editor
should be programmed as follows:
Protocol Name: H. pylori Rb
Protocol Template (Description): Rb HRP Template 1
Dewaxing (DS Option): DS2
Antigen Retrieval (AR Option): AR2, low pH; 101°C
Reagent Name, Time, Temp.: H. pylori Rb, 30 min., 37°C
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CTLA-4 clone: CAL49
Gyártó: Biocare Medical
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